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Ultra-performance liquid chromatography triple quadrupole mass spectrometry (UPLC-TQ-MS) (Xevo TQ-S system, Waters Corp) will be used to quantitatively measure the metabolites (neurotransmitters) selected as potential markers of depression. Briefly, an aliquot of 40 μl of urine or plasma will be spiked with 10 μL of internal standard (L-4-chlorophenylalanine in water, 30 μg/m L) and extracted with 200 μL of acetonitrile and methanol (9:1, v/v). The mixture will be vortexed and centrifuged.
JMIR Res Protoc 2021;10(5):e25556
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Reference 54: A 30-s chair-stand test as a measure of lower body strength in community-residing older
JMIR Res Protoc 2021;10(3):e25838
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